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pcr thermal cycler  (Eppendorf AG)


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    Structured Review

    Eppendorf AG pcr thermal cycler
    Pcr Thermal Cycler, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 99/100, based on 201118 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcr+thermal+cycler/Eppendorf/pmc13049296-59-13-16
    Average 99 stars, based on 201118 article reviews
    pcr thermal cycler - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Synthesized:

    Article Title: Investigation of the effects and underlying mechanisms of adenylate kinase 1 on inosine monophosphate deposition in Beijing-You chickens.
    Article Snippet: The concentration and quality of RNA were determined using a multimode microplate reader (Thermo Fisher Scientific, USA) and samples with an A260/A280 ratio between 1.8 and 2.0 were considered suitable for downstream applications. .. Complementary DNA (cDNA) was synthesized from 1 μg of total RNA using a PCR thermal cycler (Eppendorf, DE). .. Quantitative real-time PCR was performed using Taq SYBR Green qPCR Premix (TransGen Biotech, Beijing, China) on an AriaMx RealTime PCR System (Agilent, USA).

    Article Title: The therapeutic potential of Myoki, a novel peptide in muscle atrophy: mechanisms and applications
    Article Snippet: .. Total RNA was extracted, and complementary DNA (cDNA) samples were synthesized using a cDNA reverse transcription kit (Enzynomics, Daejeon, South Korea) according to the manufacturer’s instructions, using a PCR thermal cycler (Eppendorf, Hamburg, Germany). .. For RT-PCR, the PCR products were electrophoresed in 1.5% agarose gel containing EtBr at 100 V for 30 min and visualized using a ChemiDocTM XRS imaging system (Bio-Rad, Hercules, CA, USA).

    Article Title: Investigation of the effects and underlying mechanisms of adenylate kinase 1 on inosine monophosphate deposition in Beijing-You chickens
    Article Snippet: The concentration and quality of RNA were determined using a multimode microplate reader (Thermo Fisher Scientific, USA) and samples with an A260/A280 ratio between 1.8 and 2.0 were considered suitable for downstream applications. .. Complementary DNA (cDNA) was synthesized from 1 μg of total RNA using a PCR thermal cycler (Eppendorf, DE). .. Quantitative real-time PCR was performed using Taq SYBR Green qPCR Premix (TransGen Biotech, Beijing, China) on an AriaMx Real-Time PCR System (Agilent, USA).

    Polymerase Chain Reaction:

    Article Title: Investigation of the effects and underlying mechanisms of adenylate kinase 1 on inosine monophosphate deposition in Beijing-You chickens.
    Article Snippet: The concentration and quality of RNA were determined using a multimode microplate reader (Thermo Fisher Scientific, USA) and samples with an A260/A280 ratio between 1.8 and 2.0 were considered suitable for downstream applications. .. Complementary DNA (cDNA) was synthesized from 1 μg of total RNA using a PCR thermal cycler (Eppendorf, DE). .. Quantitative real-time PCR was performed using Taq SYBR Green qPCR Premix (TransGen Biotech, Beijing, China) on an AriaMx RealTime PCR System (Agilent, USA).

    Article Title: The therapeutic potential of Myoki, a novel peptide in muscle atrophy: mechanisms and applications
    Article Snippet: .. Total RNA was extracted, and complementary DNA (cDNA) samples were synthesized using a cDNA reverse transcription kit (Enzynomics, Daejeon, South Korea) according to the manufacturer’s instructions, using a PCR thermal cycler (Eppendorf, Hamburg, Germany). .. For RT-PCR, the PCR products were electrophoresed in 1.5% agarose gel containing EtBr at 100 V for 30 min and visualized using a ChemiDocTM XRS imaging system (Bio-Rad, Hercules, CA, USA).

    Article Title: Loss-of-function variants in ODAD1 disrupt ODA docking and induce actin cytoskeletal remodeling in primary ciliary dyskinesia
    Article Snippet: Total RNA was then reverse transcribed into cDNA with a HiScript II 1st strand cDNA synthesis kit (Vazyme, cat# R211-01). .. RT-PCR was performed on a PCR Thermal Cycler (Eppendorf) with the same initial amount of cDNA, and agarose gel electrophoresis was performed on a Wide Mini-Sub Cell GT Cell (Bio-Rad). qRT-PCR analysis was performed with HiScript Q RT SuperMix (Vazyme, cat# R223-01) using a CFX-Touch System (Bio-Rad). .. For the quantitative analysis of cilia beating, video recordings were processed using ImageJ software (National Institutes of Health, USA).

    Article Title: SFPQ directs histone H3.3 deposition to R-loops in DNA repeats to protect genome stability
    Article Snippet: .. 100 μl reactions including 10 μM of each strand were heated to 95 °C for 5 min and gradually cooled to 15 °C at a rate of 1 °C min −1 using a PCR thermal cycler (Eppendorf). ..

    Article Title: SFPQ directs histone H3.3 deposition to R-loops in DNA repeats to protect genome stability.
    Article Snippet: .. 100 μl reactions including 10 μM of each strand were heated to 95°C for 5 min and gradually cooled to 15 °C at a rate of 1°C min-1 using a PCR thermal cycler (Eppendorf). ..

    Article Title: Loss-of-function variants in ODAD1 disrupt ODA docking and induce actin cytoskeletal remodeling in primary ciliary dyskinesia.
    Article Snippet: Total RNA was then reverse transcribed into cDNA with a HiScript II 1st strand cDNA synthesis kit (Vazyme, cat# R211-01). .. RT-PCR was performed on a PCR Thermal Cycler (Eppendorf) with the same initial amount of cDNA, and agarose gel electrophoresis was performed on a Wide Mini-Sub Cell GT Cell (Bio-Rad). qRT-PCR analysis was performed with HiScript Q RT SuperMix (Vazyme, cat# R223-01) using a CFX-Touch System (Bio-Rad). ..

    Article Title: Investigation of the effects and underlying mechanisms of adenylate kinase 1 on inosine monophosphate deposition in Beijing-You chickens
    Article Snippet: The concentration and quality of RNA were determined using a multimode microplate reader (Thermo Fisher Scientific, USA) and samples with an A260/A280 ratio between 1.8 and 2.0 were considered suitable for downstream applications. .. Complementary DNA (cDNA) was synthesized from 1 μg of total RNA using a PCR thermal cycler (Eppendorf, DE). .. Quantitative real-time PCR was performed using Taq SYBR Green qPCR Premix (TransGen Biotech, Beijing, China) on an AriaMx Real-Time PCR System (Agilent, USA).

    Article Title: aFGF rescues high glucose-induced senescent fibroblasts and improves diabetic wound healing by regulating SIRT1/STAT3 pathway
    Article Snippet: Total RNA was extracted from L929 by using RNA-Quick Purification Kit (Yishan Biotechnology, RN001). .. Next, total RNA (500 ng) was reverse transcribed into cDNA by using ABScript III Reverse Transcription Mix for qPCR with gDNA remover Kit (ABclonal, RK20429) and a PCR thermal cycler (Mastercycler gradient, Eppendorf, Germany). .. The qRT-PCR assay was performed using 2X Universal SYBR Green Fast qPCR Mix (ABclonal, RK21203) and a thermal system (Mastercycler ep realplex, Eppendorf, Germany).

    Reverse Transcription:

    Article Title: The therapeutic potential of Myoki, a novel peptide in muscle atrophy: mechanisms and applications
    Article Snippet: .. Total RNA was extracted, and complementary DNA (cDNA) samples were synthesized using a cDNA reverse transcription kit (Enzynomics, Daejeon, South Korea) according to the manufacturer’s instructions, using a PCR thermal cycler (Eppendorf, Hamburg, Germany). .. For RT-PCR, the PCR products were electrophoresed in 1.5% agarose gel containing EtBr at 100 V for 30 min and visualized using a ChemiDocTM XRS imaging system (Bio-Rad, Hercules, CA, USA).

    Article Title: aFGF rescues high glucose-induced senescent fibroblasts and improves diabetic wound healing by regulating SIRT1/STAT3 pathway
    Article Snippet: Total RNA was extracted from L929 by using RNA-Quick Purification Kit (Yishan Biotechnology, RN001). .. Next, total RNA (500 ng) was reverse transcribed into cDNA by using ABScript III Reverse Transcription Mix for qPCR with gDNA remover Kit (ABclonal, RK20429) and a PCR thermal cycler (Mastercycler gradient, Eppendorf, Germany). .. The qRT-PCR assay was performed using 2X Universal SYBR Green Fast qPCR Mix (ABclonal, RK21203) and a thermal system (Mastercycler ep realplex, Eppendorf, Germany).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Loss-of-function variants in ODAD1 disrupt ODA docking and induce actin cytoskeletal remodeling in primary ciliary dyskinesia
    Article Snippet: Total RNA was then reverse transcribed into cDNA with a HiScript II 1st strand cDNA synthesis kit (Vazyme, cat# R211-01). .. RT-PCR was performed on a PCR Thermal Cycler (Eppendorf) with the same initial amount of cDNA, and agarose gel electrophoresis was performed on a Wide Mini-Sub Cell GT Cell (Bio-Rad). qRT-PCR analysis was performed with HiScript Q RT SuperMix (Vazyme, cat# R223-01) using a CFX-Touch System (Bio-Rad). .. For the quantitative analysis of cilia beating, video recordings were processed using ImageJ software (National Institutes of Health, USA).

    Article Title: Loss-of-function variants in ODAD1 disrupt ODA docking and induce actin cytoskeletal remodeling in primary ciliary dyskinesia.
    Article Snippet: Total RNA was then reverse transcribed into cDNA with a HiScript II 1st strand cDNA synthesis kit (Vazyme, cat# R211-01). .. RT-PCR was performed on a PCR Thermal Cycler (Eppendorf) with the same initial amount of cDNA, and agarose gel electrophoresis was performed on a Wide Mini-Sub Cell GT Cell (Bio-Rad). qRT-PCR analysis was performed with HiScript Q RT SuperMix (Vazyme, cat# R223-01) using a CFX-Touch System (Bio-Rad). ..

    Agarose Gel Electrophoresis:

    Article Title: Loss-of-function variants in ODAD1 disrupt ODA docking and induce actin cytoskeletal remodeling in primary ciliary dyskinesia
    Article Snippet: Total RNA was then reverse transcribed into cDNA with a HiScript II 1st strand cDNA synthesis kit (Vazyme, cat# R211-01). .. RT-PCR was performed on a PCR Thermal Cycler (Eppendorf) with the same initial amount of cDNA, and agarose gel electrophoresis was performed on a Wide Mini-Sub Cell GT Cell (Bio-Rad). qRT-PCR analysis was performed with HiScript Q RT SuperMix (Vazyme, cat# R223-01) using a CFX-Touch System (Bio-Rad). .. For the quantitative analysis of cilia beating, video recordings were processed using ImageJ software (National Institutes of Health, USA).

    Article Title: Loss-of-function variants in ODAD1 disrupt ODA docking and induce actin cytoskeletal remodeling in primary ciliary dyskinesia.
    Article Snippet: Total RNA was then reverse transcribed into cDNA with a HiScript II 1st strand cDNA synthesis kit (Vazyme, cat# R211-01). .. RT-PCR was performed on a PCR Thermal Cycler (Eppendorf) with the same initial amount of cDNA, and agarose gel electrophoresis was performed on a Wide Mini-Sub Cell GT Cell (Bio-Rad). qRT-PCR analysis was performed with HiScript Q RT SuperMix (Vazyme, cat# R223-01) using a CFX-Touch System (Bio-Rad). ..

    Quantitative RT-PCR:

    Article Title: Loss-of-function variants in ODAD1 disrupt ODA docking and induce actin cytoskeletal remodeling in primary ciliary dyskinesia
    Article Snippet: Total RNA was then reverse transcribed into cDNA with a HiScript II 1st strand cDNA synthesis kit (Vazyme, cat# R211-01). .. RT-PCR was performed on a PCR Thermal Cycler (Eppendorf) with the same initial amount of cDNA, and agarose gel electrophoresis was performed on a Wide Mini-Sub Cell GT Cell (Bio-Rad). qRT-PCR analysis was performed with HiScript Q RT SuperMix (Vazyme, cat# R223-01) using a CFX-Touch System (Bio-Rad). .. For the quantitative analysis of cilia beating, video recordings were processed using ImageJ software (National Institutes of Health, USA).

    Article Title: Loss-of-function variants in ODAD1 disrupt ODA docking and induce actin cytoskeletal remodeling in primary ciliary dyskinesia.
    Article Snippet: Total RNA was then reverse transcribed into cDNA with a HiScript II 1st strand cDNA synthesis kit (Vazyme, cat# R211-01). .. RT-PCR was performed on a PCR Thermal Cycler (Eppendorf) with the same initial amount of cDNA, and agarose gel electrophoresis was performed on a Wide Mini-Sub Cell GT Cell (Bio-Rad). qRT-PCR analysis was performed with HiScript Q RT SuperMix (Vazyme, cat# R223-01) using a CFX-Touch System (Bio-Rad). ..

    Real-time Polymerase Chain Reaction:

    Article Title: aFGF rescues high glucose-induced senescent fibroblasts and improves diabetic wound healing by regulating SIRT1/STAT3 pathway
    Article Snippet: Total RNA was extracted from L929 by using RNA-Quick Purification Kit (Yishan Biotechnology, RN001). .. Next, total RNA (500 ng) was reverse transcribed into cDNA by using ABScript III Reverse Transcription Mix for qPCR with gDNA remover Kit (ABclonal, RK20429) and a PCR thermal cycler (Mastercycler gradient, Eppendorf, Germany). .. The qRT-PCR assay was performed using 2X Universal SYBR Green Fast qPCR Mix (ABclonal, RK21203) and a thermal system (Mastercycler ep realplex, Eppendorf, Germany).



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